中文Porcine Circovirus nooca 2 xirmada ogaanshaha PCR
Magaca Alaabta
Porcine circovirus nooca 2 Qalabka Baadhista PCR (Lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Xirmadani waxay isticmaashaa habka PCR ee wakhtiga-dhabta ah si ay u ogaato RNA of Porcine circovirus type 2 (PCV2) ee qalabka cudurada unugyada sida qumanka, qanjidhada qanjidhada iyo beeryarada iyo walxaha cudurada dareeraha ah sida tallaalka iyo dhiiga.Waxay ku habboon tahay ogaanshaha, ogaanshaha iyo baadhista cudurrada faafa ee Porcine circovirus nooca 2. Qalabkani waa ALL-DIYAAR PCR NIDAAMKA(Lyophilized), kaas oo ka kooban enzym-weyneynta DNA-ga, falcelinta falcelinta, curiyeyaasha gaarka ah iyo baaritaannada looga baahan yahay ogaanshaha PCR ee fluorescent. .
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku-dhafka PCV2 PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baaritaanno,Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus PCV2 oo ka kooban jajabyo gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 95 | 2 daqiiqo | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Ururi foornada FAM |
* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Vibrio Parahaemolyticus togan |
| Undet | Vibrio Parahaemolyticus Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
