Qalabka lagu ogaado cudurka 'Pseudorabies virus (gB) PCR'

Sharax Gaaban:

Qalabkani wuxuu isticmaalaa habka dhabta ah ee loo yaqaan 'PCR fluorescent PCR' si loo ogaado RNA ee fayraska 'Pseudorabies virus' (gB gen) '(PRV) ee qalabka cudurada unugyada sida qumanka, qanjidhada qanjirada iyo beeryarada iyo qalabka cudurrada dareeraha ah sida tallaalka iyo dhiigga doofaarrada.


Faahfaahinta alaabta

Calaamadaha alaabta

Magaca Badeecada

Fayraska Pseudorabies (gB) Qalabka baaritaanka PCR (Lyophilized)

Cabir

48 banaanbax / xirmo, 50 mudaaharaad / xirmo

Isticmaalka loogu talo galay

Qalabkani wuxuu isticmaalaa habka dhabta ah ee loo yaqaan 'PCR fluorescent PCR' si loo ogaado RNA ee fayraska 'Pseudorabies virus' (gB gen) '(PRV) ee qalabka cudurada unugyada sida qumanka, qanjidhada qanjirada iyo beeryarada iyo qalabka cudurrada dareeraha ah sida tallaalka iyo dhiigga doofaarrada. Waxay ku habboon tahay ogaanshaha, ogaanshaha iyo baaritaanka cudurrada faafa ee fayraska 'Pseudorabies virus' (gB gene). Xirmadaani waa DHAMMAAN-DIYAARSAN NIDAAMKA PCR (Lyophilized), oo ay kujirto dheecaanka dheecaanka DNA-da, falcelinta keydka, astaamo gaar ah iyo baaritaanno looga baahan yahay ogaanshaha dareeraha RT-PCR.

Tusmada alaabta

Qaybaha Xirmo faahfaahinta Waxyaabaha ay ka kooban tahay
Isku-darka PRV PCR 1 × dhalo (budada Lyophilized)  50Baaritaan dNTPs, MgCl2, Horudhac, Baaritaanno, Taq DNA polymerase
6 × 0.2ml 8 tuubo sifiican u fiiqan(Liophilized) 48Tijaabinta
Xakamaynta wanaagsan 1 * 0.2ml tuubbo (liophilized)  10Tijaabooyin

Plasmid ama Pseudovirus ka kooban jajab gaar ah PRV (gB)

Xallinta xalka 1.5 ml Cryotube 500uL /
Xakamaynta xun 1.5 ml Cryotube 200uL 0.9% NaCl

Kaydinta & Nolosha shelf

(1) Xidhmada waxaa lagu qaadi karaa heerkulka qolka.

(2) Nolosha shelf waa 18 bilood oo ah -20 ℃ iyo 12 bilood oo ah 2 ℃ ~ 30 ℃.

(3) Eeg calaamadda ku qoran xirmada taariikhda soo-saarka iyo taariikhda uu dhacayo.

(4) Nooca reagent ee budada lyophilized waa in lagu kaydiyaa -20 ℃ ka dib kala dirista iyo qaboojinta soo noqnoqota - dhalaalku waa inuu ka yaraadaa 4 jeer.

Qalab

GENECHECKER UF-150, UF-300 waqtiga dhabta ah qalabka PCR fluorescence.

Jaantuska Hawlgalka

a) Nooca dhalada:

1

b) 8 nooc tuubbo sifiican u-fiiqan:

2

Kordhinta PCc

Dejinta lagu taliyay

Tallaabada

Wareeg

Heerkulka (℃)

Waqti

Kanaalka iftiinka

1

1

95

2 min

/

2

40

95

5s

/

60

10s

Ururi FAM fluorescence

* Xusuusin: Calaamadaha kanaalada FAM fluorescence-ka waxaa lagu soo ururin doonaa 60 ℃.

Turjumida Natiijooyinka Imtixaanka

Kanaalka

Fasiraadda natiijooyinka

Kanaalka FAM

Ct≤35

PRV (gB) Wanaagsan

Undet

PRV (gB) xun

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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