E.coli O157: qalabka baarista H7 PCR

Sharax Gaaban:

Colcher O157: H7 (E.coli O157: H7) waa bakteeriya gram-negative ah oo ka tirsan genus Enterobacteriaceae, oo soo saarta tiro badan oo sunta Vero ah.


Faahfaahinta alaabta

Calaamadaha alaabta

Magaca Badeecada

E.coli O157: Qalabka baarista H7 PCR (Lyophilized)

Cabir

48 banaanbax / xirmo, 50 mudaaharaad / xirmo

Isticmaalka loogu talo galay

Colcher O157: H7 (E.coli O157: H7) waa bakteeriya gram-negative ah oo ka tirsan genus Enterobacteriaceae, oo soo saarta tiro badan oo sunta Vero ah. Caafimaad ahaan, badanaa waxay si lama filaan ah ugu dhacdaa calool xanuun daran iyo shuban-biyood, waxaana ku xiga shuban-biyoodka dhowr maalmood ka dib, kaasoo u horseedi kara qandho ama qandho la'aan, iyo dhimasho xaaladaha daran. Qalabkani wuxuu ku habboon yahay ogaanshaha tayada Escherichia coli O157: H7 ee cuntada, shaybaarka biyaha, saxarada, matagga, dareeraha xoojiya bakteeriyada iyo shaybaarada kale iyadoo la adeegsanayo mabda'a waqtiga dhabta ah ee PCR. Lyophilized), oo ay kujirto dheecaanka dheecaanka DNA-ga, falcelinta keydka, astaamo gaar ah iyo baaritaanno looga baahan yahay ogaanshaha PCR fluorescent ..

Tusmada alaabta

Qaybaha Xirmo faahfaahinta Waxyaabaha ay ka kooban tahay
E.coli O157: H7 PCR Isku Darka 1 × dhalo (budada Lyophilized)  50Baaritaan dNTPs, MgCl2, Horudhac, Baadhitaano, Dib-u-Celin Qoraal, Taq DNA polymerase
6 × 0.2ml 8 tuubo sifiican u fiiqan(Liophilized) 48Tijaabinta
Xakamaynta wanaagsan 1 * 0.2ml tuubbo (liophilized)  10Tijaabooyin

Plasmid oo ka kooban E.coli O157: H7 jajabyo gaar ah

Xallinta xalka 1.5 ml Cryotube 500uL /
Xakamaynta xun 1.5 ml Cryotube 200uL 0.9% NaCl

Kaydinta & Nolosha shelf

(1) Xidhmada waxaa lagu qaadi karaa heerkulka qolka.

(2) Nolosha shelf waa 18 bilood oo ah -20 ℃ iyo 12 bilood oo ah 2 ℃ ~ 30 ℃.

(3) Eeg calaamadda ku qoran xirmada taariikhda soo-saarka iyo taariikhda uu dhacayo.

(4) Nooca reagent ee budada lyophilized waa in lagu kaydiyaa -20 ℃ ka dib kala dirista iyo qaboojinta soo noqnoqota - dhalaalku waa inuu ka yaraadaa 4 jeer.

Qalab

GENECHECKER UF-150, UF-300 waqtiga dhabta ah qalabka PCR fluorescence.

Jaantuska Hawlgalka

a) Nooca dhalada:

1

b) 8 nooc tuubbo sifiican u-fiiqan:

2

Kordhinta PCc

Lagu taliyay Dejinta

Tallaabada     Wareeg Heerkulka (℃)   Waqti Kanaalka iftiinka
1 1 95 2 min  
2 40 95 5s  
60 10s Ururi FAM fluorescence

* Xusuusin: Calaamadda kanaalka FAM fluorescence waxaa lagu ururin doonaa 60 ℃.

Turjumida Natiijooyinka Imtixaanka

Kanaalka

Fasiraadda natiijooyinka

Kanaalka FAM

Ct≤35

E.coli O157: H7 Wanaagsan

Undet

E.coli O157: H7 taban

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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