中文E.coli O157:H7 xirmada ogaanshaha PCR
Magaca Alaabta
E.coli O157:H7 Qalabka ogaanshaha PCR (Lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Escherichia coli O157:H7 (E.coli O157:H7) waa bakteeriya garaam-negative ah oo ka tirsan genus Enterobacteriaceae, taasoo soo saarta xaddi badan oo sunta Vero ah.Caafimaad ahaan, inta badan waxa ay si lama filaan ah ugu dhacdaa calool xanuun aad u daran iyo shuban biyood, waxa ku xiga shuban dhiig baxa ah maalmo ka dib, kaas oo keeni kara qandho ama qandho la’aan, iyo dhimasho xaalado daran.Qalabkani wuxuu ku habboon yahay in lagu ogaado tayada Escherichia coli O157:H7 ee cuntada, shaybaarada biyaha, saxarada, matagga, dareeraha bakteeriyada xoojiya iyo muunado kale iyadoo la adeegsanayo mabda'a PCR-da dhabta ah. Lyophilized), oo ka kooban enzym-weyneynta DNA-ga, falcelinta falcelinta, furayaasha gaarka ah iyo baaritaannada looga baahan yahay ogaanshaha PCR.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| E.coli O157:H7 PCR Mix | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baadhitaanno, Dib-u-eegis Qoraal, Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ka kooban E.coli O157:H7 jajabyo gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
lagu taliyayDejinta
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 95 | 2 daqiiqo | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Ururi foornada FAM |
* Xusuusin: Calaamadaha kanaalka FAM fluorescence waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | E.coli O157:H7 Wanaagsan |
| Undet | E.coli O157:H7 Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
