中文Qalabka lagu ogaado Salmonella PCR
Magaca Alaabta
Qalabka lagu ogaado Salmonella PCR (lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Salmonella waxaa iska leh Enterobacteriaceae iyo Gram-negative enterobacteria.Salmonella waa cudur-sidaha cuntada ka dhasha ee caadiga ah wuxuuna ku jiraa marka ugu horreysa ee sumowga cuntada.Calaamadaha ugu waaweyn ee sumowga cuntada ee uu keeno Salmonella waa lallabbo, matag, calool xanuun, madax-xanuun, qarqaryo iyo shuban.Xirmadani waxay isticmaashaa mabda'a PCR-ga ee fluorescent-ka-dhabta ah waxayna ku habboon tahay in lagu ogaado tayada Salmonella ee cuntada, muunadaha biyaha, saxarada, matagga, iyo dareeraha kobcinta.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku-dhafka PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baaritaanno,Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus oo ka kooban jajab gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 100ul | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 95 | 2 daqiiqo | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Ururi foornada FAM |
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Salmonella Togan |
| Undet | Salmonella Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
