中文Qalabka ogaanshaha ee PCR Shigella
Magaca Alaabta
Qalabka ogaanshaha PCR ee Shigella (lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Shigella waa nooc ka mid ah bacilli gram-negative , oo ka tirsan cudurada mindhicirrada, iyo cudur-gaare ugu caansan ee dysentery bacillary aadanaha.Xirmadani waxay isticmaashaa mabda'a PCR-ka-dhaliyaha-waqtiga-dhabta ah, kaas oo ku habboon go'aaminta Shigella ee cuntada, muunadaha biyaha, saxarada, matagga, iyo dareeraha kobcinta.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku-dhafka PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baaritaanno,Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus oo ka kooban jajab gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 100ul | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 95 | 2 daqiiqo | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Ururi foornada FAM |
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Shigella Togan |
| Undet | Shigella Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
