中文Qalabka ogaanshaha PCR ee Listeria monocytogenes
Magaca Alaabta
Qalabka ogaanshaha PCR ee Listeria monocytogenes
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Listeria monocytogenes waa microbacterium garaam-positive ah oo kori kara inta u dhaxaysa 4 ℃ iyo 45 ℃.Waa mid ka mid ah cudurada ugu waaweyn ee khatarta ku ah caafimaadka aadanaha ee ku jira cuntada qaboojiyaha.Calaamadaha ugu muhiimsan ee caabuqa waa septicemia, meningitis iyo mononucleosis.Qalabkani wuxuu ku haboon yahay in lagu ogaado tayada Listeria monocytogenes ee cuntada, shaybaarada biyaha, saxarada, matagga, dareeraha bakteeriyada xoojiya iyo muunado kale iyadoo la adeegsanayo mabda'a waqtiga-dhabta ah ee PCR , kaas oo ka kooban enzym-weyneynta DNA-ga, transcriptase-ka-noqoshada, fal-celinta falcelinta, curiyeyaasha gaarka ah iyo baarayaasha looga baahan yahay ogaanshaha PCR ee fluorescent.
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Listeria monocytogenes PCR Mix | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baadhitaanno, Dib-u-eegis Qoraal, Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ka kooban Listeria monocytogenes qaybo gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a)
b)
Kordhinta PCr
lagu taliyayDejinta
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 50 | 8 daq | |
| 2 | 1 | 95 | 2 daqiiqo | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Ururi foornada FAM |
* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Listeria monocytogenes togan |
| Undet | Listeria monocytogenes Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
