Xirmada ogaanshaha ee RT-PCR

Sharaxaad Gaaban:

Xirmadani waxay isticmaashaa habka fluorescent RT-PCR ee wakhtiga-dhabta ah si ay u ogaato RNA ee Porcine taranka iyo neefsiga syndrome-ka xirmada ogaanshaha fayraska nucleic acid (PRRSV) ee qalabka cudurada unugyada sida qumanka, qanjidhada qanjidhada iyo beeryarada iyo walxaha cudurada dareeraha ah sida tallaalka iyo dhiiga doofaarrada .


Faahfaahinta Alaabta

Tags Product

Magaca Alaabta

Xirmada ogaanshaha ee RT-PCR (Lyophilized) ee fayraska taranka iyo neef-mareenka

Cabbirka

48tijaab/xirmo, 50tijaab/xirmo

Isticmaalka la damacsan yahay

Xirmadani waxay isticmaashaa habka fluorescent RT-PCR ee wakhtiga-dhabta ah si ay u ogaato RNA ee Porcine taranka iyo neefsiga syndrome-ka xirmada ogaanshaha fayraska nucleic acid (PRRSV) ee qalabka cudurada unugyada sida qumanka, qanjidhada qanjidhada iyo beeryarada iyo walxaha cudurada dareeraha ah sida tallaalka iyo dhiiga doofaarrada .Waxay ku habboon tahay ogaanshaha, ogaanshaha iyo baadhista cudurrada faafa ee Fayraska Dhegaha Buluugga ah ee Porcine.Qalabku waa NIDAAMKA PCR-DIYAAR AH (Lyophilized), kaas oo ka kooban transcriptase-ka-noqoshada, enzym-weynida DNA-ga, falcelinta falcelinta, furayaasha gaarka ah iyo baarayaasha loo baahan yahay si loo ogaado fluorescent RT-PCR.

Nuxurka Alaabta

Qaybaha Xidhmada qeexid Walaxda
Isku dhafka PBEV PCR 1 × dhalo (budada Lyophilized)  50Tijaabi dNTPs, MgCl2, Aasaaska, Baadhitaanno, Dib-u-eegis Qoraal, Taq DNA polymerase
6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) 48Tijaabi
Xakamaynta togan 1 * 0.2ml tuubo (lyophilized)  10Tijaabooyin

Plasmid ama Pseudovirus ka kooban PRRSV jajabyo gaar ah

Xalka kala dirida 1.5 ml Cryotube 500uL /
Xakamaynta taban 1.5 ml Cryotube 200uL 0.9% NaCl

Kaydinta & Nolosha shelf

(1) Qalabka waxa lagu rari karaa heerkulka qolka.

(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.

(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.

(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.

Qalabka

GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.

Shaxda hawlgalka

a) Nooca dhalada:

1

b) 8 nooca tubbada si fiican loo jaro:

2

Kordhinta PCr

Dejinta lagu taliyay

Talaabada

Wareegto

Heerkulka (℃)

Waqtiga

Kanaalka fluorescence

1

1

48

8 daq

/

2

1

95

2 daqiiqo

/

3

40

95

5s

/

60

10s

Ururi foornada FAM

* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.

Turjumaada Natiijooyinka Imtixaanka

Kanaalka

Fasiraadda natiijooyinka

FAM Channel

Ct≤35

Vibrio Parahaemolyticus togan

Undet

Vibrio Parahaemolyticus Negative

35

Natiijada shakiga leh, dib u tijaabi*

*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.


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