中文Xirmada ogaanshaha fayraska shuban biyoodka ee RT-PCR
Magaca Alaabta
Xirmada ogaanshaha ee RT-PCR (Lyophilized) ee cudurka shuban biyoodka
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Xirmadani waxay isticmaashaa habka fluorescent RT-PCR ee wakhtiga-dhabta ah si ay u ogaato RNA ee fayraska shuban-biyoodka faafa (PEDV) ee ku jira qalabka cudurada unugyada sida qumanka, qanjidhada qanjidhada iyo beeryarada iyo walxaha cudurada dareeraha ah sida tallaalka iyo dhiiga doofaarka.Waxay ku habboon tahay ogaanshaha, ogaanshaha iyo baaritaanka cudurrada faafa ee fayraska shuban-biyoodka ee Porcine.Qalabku waa ALL-DIYAAR PCR NIDAAMKA (Lyophilized), kaas oo ka kooban transcriptase-ka-noqoshada, enzym-weynida DNA-ga, falcelinta falcelinta, aasaasiyaal gaar ah iyo baarayaal looga baahan yahay ogaanshaha RT-PCR.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku dhafka PEDV PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baaritaanno,Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus oo ka kooban qaybo gaar ah oo PEDV ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 48 | 8 daq | / |
| 2 | 1 | 95 | 2 daqiiqo | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Ururi foornada FAM |
* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | PEDV Wanaagsan |
| Undet | PEDV Negative |
| 35 | Natiijo shaki leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
