中文Xirmada ogaanshaha ee RT-PCR
Magaca Alaabta
Xirmada ogaanshaha ee RT-PCR (Lyophilized) ee fayraska taranka iyo neef-mareenka
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Xirmadani waxay isticmaashaa habka fluorescent RT-PCR ee wakhtiga-dhabta ah si ay u ogaato RNA ee Porcine taranka iyo neefsiga syndrome-ka xirmada ogaanshaha fayraska nucleic acid (PRRSV) ee qalabka cudurada unugyada sida qumanka, qanjidhada qanjidhada iyo beeryarada iyo walxaha cudurada dareeraha ah sida tallaalka iyo dhiiga doofaarrada .Waxay ku habboon tahay ogaanshaha, ogaanshaha iyo baadhista cudurrada faafa ee Fayraska Dhegaha Buluugga ah ee Porcine.Qalabku waa NIDAAMKA PCR-DIYAAR AH (Lyophilized), kaas oo ka kooban transcriptase-ka-noqoshada, enzym-weynida DNA-ga, falcelinta falcelinta, furayaasha gaarka ah iyo baarayaasha loo baahan yahay si loo ogaado fluorescent RT-PCR.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku dhafka PBEV PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baadhitaanno, Dib-u-eegis Qoraal, Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus ka kooban PRRSV jajabyo gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 48 | 8 daq | / |
| 2 | 1 | 95 | 2 daqiiqo | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Ururi foornada FAM |
* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Vibrio Parahaemolyticus togan |
| Undet | Vibrio Parahaemolyticus Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
