中文Qalabka lagu ogaanayo xumadda doofaarka ee RT-PCR
Magaca Alaabta
Qalabka lagu ogaado qandhada doofaarka ee RT-PCR (Lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Xirmadani waxay isticmaashaa habka fluorescent RT-PCR ee wakhtiga-dhabta ah si ay u ogaato RNA ee fayraska qandhada Doofaarka (CSFV) ee walxaha cudurada unugyada sida qumanka, qanjidhada qanjidhada iyo beeryarada iyo walxaha cudurada dareeraha ah sida tallaalka iyo dhiiga doofaarka.Waxay ku habboon tahay ogaanshaha, ogaanshaha iyo baadhista cudurrada faafa ee fayraska qandhada doofaarka.Qalabku waa NIDAAMKA PCR-DIYAARAHA DHAMMAAN (Lyophilized), kaas oo ka kooban enzym-weynaysiinta DNA-ga, beddelka transcriptase, falcelinta falcelinta, aasaasiyaal gaar ah iyo baarayaal looga baahan yahay ogaanshaha PCR ee fluorescent.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku-dhafka CSFV PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baadhitaanno, Dib-u-eegis Qoraal, Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ka kooban qaybo gaar ah oo CSFV ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
lagu taliyayDejinta
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 50 | 8 daq | |
| 2 | 1 | 95 | 2 daqiiqo | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Ururi foornada FAM |
* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | CSFV Wanaagsan |
| Undet | CSFV Negative |
| 35 | Natiijo shaki leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
