中文Qalabka lagu ogaado Staphylococcus aureus PCR
Magaca Alaabta
Staphylococcus aureus Qalabka ogaanshaha PCR (lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Staphylococcus aureus waxaa iska leh genus Staphylococcus waana bakteeriyada garaam-positive ah.Waa unug-noole-jiif oo caadi ah oo ka dhasha cunnada taasoo soo saari karta enterotoxins oo keeni karta cunto ku-sumo.Xirmadani waxay isticmaashaa mabda'a PCR-ga ee fluorescent-ka-dhabta ah waxayna ku habboon tahay in lagu ogaado tayada Staphylococcus aureus ee cuntada, muunadaha biyaha, saxarada, matagga, dareeraha kobcinta iyo muunado kale.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku-dhafka PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baaritaanno,Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus oo ka kooban jajab gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 100ul | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 95 | 2 daqiiqo | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Ururi foornada FAM |
Turjumaada Natiijooyinka Imtixaanka
| Channel | Fasiraadda natiijooyinka |
| FAMChannel | |
| Ct≤35 | Staphylococcus aureus Wanagsan |
| Undet | Staphylococcus aureus xun |
| 35<Ct≤40 | Shakinatiijada,dib u tijaabi* |
*Haddiiah natiijada ku celiska ah ee kanaalka FAMhasqiimaha Ct ≤40waxayna muujinaysaa caadi"S”qalooca dhawaaqa qaabaynta, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
