中文Qalabka ogaanshaha ee Vibrio parahaemolyticus PCR
Magaca Alaabta
Vibrio Parahaemolyticus PCR Xirmada Baadhista (Lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Vibrio Parahemolyticus (sidoo kale loo yaqaan Halophile Vibrio Parahemolyticus) waa bacillus polymorphic Gram-negative ama Vibrio Parahemolyticus. oo leh bilaw ba'an, calool xanuun, matag, shuban iyo saxaro biyood oo ah calaamadaha ugu muhiimsan ee kiliiniga. PCR oo ku habboon in la ogaado tayada Vibrio Parahaemolyticus ee cuntada, shaybaarada biyaha, saxarada, matagga, iyo dheecaanka kobcinta.Qalabka waa ALL-DIYAAR PCR NIDAAMKA iyo baarayaal looga baahan yahay ogaanshaha RT-PCR fluorescent.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Isku-dhafka PCR | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baaritaanno,Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ama Pseudovirus oo ka kooban jajab gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 100ul | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
Dejinta lagu taliyay
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 95 | 2 daqiiqo | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Ururi foornada FAM |
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Vibrio Parahaemolyticus togan |
| Undet | Vibrio Parahaemolyticus Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
