中文Qalabka lagu ogaado Norovirus (GⅠ) RT-PCR
Magaca Alaabta
Norovirus (GⅠ) Qalabka Baadhista RT-PCR (Lyophilized)
Cabbirka
48tijaab/xirmo, 50tijaab/xirmo
Isticmaalka la damacsan yahay
Waxay ku habboon tahay in la ogaado Norovirus (GⅠ) ee kalluunka qolofleyda ah, khudaarta iyo miraha ceeriin, biyaha, saxarada, matagga iyo noocyo kale.Soo saarista aashitada nucleic-ka waa in lagu fuliyaa xirmada soo saarista aashitada nucleic ama habka pyrolysis tooska ah iyadoo loo eegayo noocyada kala duwan ee shaybaarka.Qorshaha waa ALL-DIYAAR PCR NIDAAMKA iyo baaritaanada looga baahan yahay ogaanshaha PCR fluorescent.
Nuxurka Alaabta
| Qaybaha | Xidhmada | qeexid | Walaxda |
| Norovirus (GⅠ) PCR Mix | 1 × dhalo (budada Lyophilized) | 50Tijaabi | dNTPs, MgCl2, Aasaaska, Baadhitaanno, Dib-u-eegis Qoraal, Taq DNA polymerase |
| 6 × 0.2ml 8 tuubo si fiican u dhuuban(Lyophilized) | 48Tijaabi | ||
| Xakamaynta togan | 1 * 0.2ml tuubo (lyophilized) | 10Tijaabooyin | Plasmid ka kooban Norovirus (GⅠ) jajabyo gaar ah |
| Xalka kala dirida | 1.5 ml Cryotube | 500uL | / |
| Xakamaynta taban | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Kaydinta & Nolosha shelf
(1) Qalabka waxa lagu rari karaa heerkulka qolka.
(2) Nolosha shelf waa 18 bilood at -20 ℃ iyo 12 bilood at 2 ℃ ~ 30 ℃.
(3) Fiiri calaamadda xirmada ku qoran taariikhda wax-soo-saarka iyo taariikhda uu dhacayo.
(4) Nooca budada lyophilized reagent waa in lagu kaydiyaa -20 ℃ ka dib kala diri iyo baraf soo noqnoqda -dhalaalaan waa in ay ahaataa wax ka yar 4 jeer.
Qalabka
GENECHECKER UF-150, UF-300 qalabka PCR ee wakhtiga-dhabta ah fluorescence.
Shaxda hawlgalka
a) Nooca dhalada:
b) 8 nooca tubbada si fiican loo jaro:
Kordhinta PCr
lagu taliyayDejinta
| Talaabada | Wareegto | Heerkulka (℃) | Waqtiga | Kanaalka fluorescence |
| 1 | 1 | 50 | 8 daq | |
| 2 | 1 | 95 | 2 daqiiqo | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Ururi foornada FAM |
* Fiiro gaar ah: Calaamadaha kanaalada Fluorescence ee FAM waxaa lagu soo ururin doonaa 60 ℃.
Turjumaada Natiijooyinka Imtixaanka
| Kanaalka | Fasiraadda natiijooyinka |
| FAM Channel | |
| Ct≤35 | Norovirus (GⅠ) Wanaagsan |
| Undet | Norovirus (GⅠ) Negative |
| 35 | Natiijada shakiga leh, dib u tijaabi* |
*Haddii natiijada ku celiska ah ee kanaalka FAM leedahay Ct qiimaha ≤40 oo ay muujiso "S" qaab qalooca caadiga ah, natiijadu waxa loo fasiraa mid togan, haddii kale waa taban.
